Clinical applications:

Supports Collagen Production
Supports Integrity of Connective Tissues
Supports Muscle Recovery Following Overwork or Exercise
Supports Muscle Health
Supports Increased Protein Synthesis and Decreased Protein Degradation

Description:

FIT Lean Food Collagen features bovine collagen peptides complemented with beta-hydroxy-beta-methylbutyrate (HMB) and vitamin D3, making it an optimal choice for supporting collagen production, the integrity of connective tissues, and muscle health. A natural metabolite of leucine, HMB increases lean muscle mass through dual mechanisms: inhibiting protein degradation and promoting protein synthesis. This versatile formula rapidly disperses in water and is ideal for athletes or for individuals who need an easily digested protein source.

Collagen Peptides

Collagen is a primary structural component of connective tissue that

accounts for as much as 30% of the body’s total protein.[1] It is found in

skin, ligaments, tendons, cartilage, tooth dentin, bone, and muscle, and it

is also part of the structure of arteries and veins. Collagen protein has a

unique amino acid composition with an unusually high content of glycine

and proline along with the modified amino acid hydroxyproline, making it

ideal for supporting the turnover and renewal of structural proteins.*[2]

Due to the well-established function of collagen’s contribution to the

integrity of articular cartilage, supplemental collagen peptides have been

studied for their potential benefit to cartilage health. The results of a

randomized, double-blind, placebo-controlled study of subjects

(N = 30) with osteoarthritis (OA) who were given placebo or 5 g of

collagen peptide twice daily for 13 weeks demonstrated that collagen

peptides were effective in improving OA symptoms. The researchers

hypothesized that the mechanism for this effect is attributed to the role

that collagen peptides play in regulating chondrocyte differentiation and

stimulating proteoglycan synthesis, resulting in cartilage tissue support.*[2]

Several studies have explored the effects of collagen peptide

supplementation on body composition, strength, and muscle health

when combined with resistance exercise. In a randomized, double-blind,

placebo-controlled study of recreationally active male subjects (N = 57),

the test group was supplemented with 15 g of collagen peptides while

engaging in a resistance training program. Both groups showed a slight

increase in strength, but an increase in fat-free mass was observed in

the supplemented group, suggesting an increased effectiveness when

combined with physical activity. Muscle fiber biopsies in subjects did

not differ between the test or placebo group, leading the researchers to

attribute the increase of fat-free mass in the supplemented group with

hypertrophy of contractile muscle cells and possibly a higher increase in

connective tissue.*[1]

Many of the studies assessing the effect of collagen protein

supplementation combined with resistance training have been conducted

with male subjects. However, a 12-week randomized, controlled trial in

pre-menopausal subjects (N = 77) showed an increase in muscle strength

and fat-free mass in women who combined 15 g of collagen peptide

supplementation with resistance exercise three-times per week.*[3]

HMB

Beta-hydroxy-beta-methylbutyrate (HMB) is a metabolite of the essential

amino acid leucine found naturally within human muscle cells, and it has

been reported to have anabolic effects on protein muscle metabolism.[4,5]

Several mechanisms of action have been proposed to explain the action

of HMB on muscle health. Among them are that HMB attenuates training-

induced proteolysis via downregulation of proteolytic pathways, and it acts

as a signaling molecule for stimulation of protein synthesis.[5-7] Studies

conducted mostly in athletes have suggested that HMB supplementation

may exert beneficial effects on muscle mass and function, most notably

when combined with resistance-type exercise training.*[6,8]

In a randomized, double-blind, placebo-controlled, parallel design study,

subjects (N = 31) aged 70 and older were given 3 g of HMB or placebo

daily for eight weeks with a prescribed exercise program for five days

per week. In the supplemented group, subjects showed an increase in

lean body mass similar to that seen in younger adults, which reported a

decrease in muscle proteolysis and an increase in fat-free mass in young

adults with resistance training. The researchers suggested that the results

are indicative of the role HMB plays in decreasing proteolysis.*[9]

Results from a randomized study in young, healthy, sedentary subjects

(N = 37) given 3 g of supplemental HMB daily for three weeks showed an

increase in fat-free mass and total body strength suggesting that HMB

partially attenuates muscle protein breakdown without exercise.[10] To

further illustrate the benefit of HMB supplementation on muscle health

without any form of exercise, a randomized, double-blind, placebo-

controlled, parallel design study was conducted with healthy subjects

(N = 24) aged 60-76 years who were confined to complete bed rest.

Subjects in the test group were given 3 g of HMB five days prior to

*These statements have not been evaluated by the Food and Drug Administration.

This product is not intended to diagnose, treat, cure, or prevent any disease.

XYMOGEN® Exclusive Professional Formulas

800-647-6100 | www.xymogen.com

Body Composition Joint & Muscle Support

Sports NutritionBody Composition

Joint & Muscle Support

Sports Nutrition

FIT Food® Lean Collagen Mocha Latte Supplement Facts

bed rest until the end of the 10-day rehabilitation phase, resulting in

the preservation of muscle mass when compared to the placebo group.

Serving Size: 1 Scoop (about 28 g) Servings Per Container: About 14

Results suggested that HMB helped to preserve muscle mass during the

immobilization period.*[11]

Several systematic reviews and meta-analyses have helped to solidify the

body of evidence supporting the effectiveness of HMB on strength, body

composition, and muscle health in trained and untrained individuals. A

meta-analysis of randomized, placebo-controlled trials with supplemental

HMB in doses ranging from 1.5 to 6 g/day for a three- to nine-week period

indicated sufficient data to support strength gains in untrained participants

and a negligible effect in trained individuals but with an inconsequential

effect on body composition.[5] A meta-analysis exploring the potential benefit

of supplements on lean mass and strength with resistance training reviewed

over 250 different supplemental ingredients and found that only six had

adequate research for inclusion. Nine HMB studies qualified with analysis

demonstrating that a 3 g/day dose provided support for net gain in strength

and lean mass gains.[12] The results of a systematic review and meta-analysis

assessing the outcome of HMB supplementation in a clinical setting of

subjects (N = 2,137) with conditions characterized by loss in skeletal muscle

mass and weakness were in line with previous reviews of older adults and

athletes, suggesting that the effect of HMB increases skeletal muscle mass

and improves muscle strength.*[13]

Amount Per Serving %Daily Value

Calories 100

Total Fat 2 g 3%†

Saturated Fat 0.5 g 3%†

Total Carbohydrate 5 g 2%†

Dietary Fiber 1 g 4%

Protein (from bovine collagen peptides) 15 g

Vitamin D3 (cholecalciferol) 12.5 mcg 63%

Calcium (from calcium beta-hydroxy-beta-methylbutyrate-

400 mg 31%

monohydrateS1 and ingredients with naturally occurring

calcium)

Sodium (naturally occurring) 120 mg 5%

Calcium Beta-Hydroxy-Beta-Methylbutyrate-MonohydrateS1 3 g **

† Percent Daily Values are based on a 2,000 calorie diet.

** Daily Value not established.

Vitamin D

Vitamin D has a well-known function in bone health due to its role in the

regulation of calcium and phosphate homeostasis. It has also been linked to

skeletal muscle health and function.[14,15] Low serum levels of vitamin D are

correlated with muscle weakness and myopathy. In vitro studies have shown

that vitamin D is necessary for the mitochondrial function of providing energy

to skeletal muscle cells.*[16]

A cross-sectional study examining the relationship between vitamin D status

and strength in collegiate athletes (N = 103) showed that a decrease in

serum D was correlated with a decrease in indicators of muscle strength and

anaerobic power. The authors concluded that vitamin D should be considered

as a component of training to maximize performance in sports requiring

muscular strength.*[17]

A systematic review and meta-analysis of 17 randomized-controlled trials

assessing vitamin D supplementation and its role in muscle strength

suggested that in individuals with baseline vitamin D levels the outcome of

supplementation was not significant, but an increase in strength was shown

in those who were deficient.[14] Another study analyzed the role of vitamin D

in muscle function with data from 30 randomized trials involving over 5,600

participants indicating that supplementation has a positive impact on muscle

strength. The authors suggested that additional research is needed to define

optimal dose, duration, and mode of administration.*[15]

There have also been studies on the combined effect of vitamin D with HMB.

In a randomized, double-blind, placebo-controlled, 12-month trial in older

subjects (N = 117), HMB (3 g) and vitamin D (2,000 IU) were shown to be of

benefit to muscular function with or without resistance training.[18] Another

study looked at previous data of vitamin D status in older adults who used

HMB supplements. It was found that muscle mass increased regardless of

vitamin D status, but strength increases were observed only in individuals

with adequate vitamin D status, demonstrating a synergistic effect of HMB

and vitamin D on muscle function.*[19]

Other Ingredients: Natural flavors (no MSG), sunflower oil, cocoa powder, silica, medium-chain

triglyceride oil, cellulose gum, xanthan gum, and monk fruit extract.

DIRECTIONS: Mix one level scoop (28 g) with 6-8 oz of cold water and

consume daily, or use as directed by your healthcare professional. Adjust

the amount of water according to thickness desired.

Consult your healthcare professional before use. Individuals taking

medication should discuss potential interactions with their healthcare

professional. Do not use if tamper seal is damaged.

STORAGE: Keep closed in a cool, dry place out of reach of children.

FORMULATED TO EXCLUDE: Wheat, gluten, yeast, soy protein, dairy

products, fish, shell fish, peanuts, tree nuts, egg, sesame, artifi cial colors,

and artificial sweeteners.

S1. myHMB® is a registered trademark of Metabolic Technologies, LLC. Nutritional uses of calcium beta-hydroxy-beta-methylbutyrate-

monohydrate and vitamin D are licensed to Metabolic Technologies, LLC, under U.S. patents 8,815,280, 9,259,430, and 9,539,224.

References

1. Kirmse M, Oertzen-Hagemann V, de Marées M, et al. Nutrients.

2019;11(5):1154. doi:10.3390/nu11051154

2. Kumar S, Sugihara F, Suzuki K, et al. J Sci Food Agric. 2015;95(4):702-707.

doi:10.1002/jsfa.6752

3. Jendricke P, Centner C, Zdzieblik D, et al. Nutrients. 2019;11(4):892.

doi:10.3390/nu11040892

4. Holeček M. J Cachexia Sarcopenia Muscle. 2017;8(4):529-541. doi:10.1002/

jcsm.12208

5. Rowlands DS, Thomson JS. J Strength Cond Res. 2009;23(3):836-846.

doi:10.1519/JSC.0b013e3181a00c80

6. Albert FJ, Morente-Sánchez J, Ortega FB, et al. Nutr Hosp. 2015;32(1):20-33.

doi:10.3305/nh.2015.32.1.9101

7. Courel-Ibáñez J, Vetrovsky T, Dadova K, et al. Nutrients. 2019;11(9):2082.

doi:10.3390/nu11092082

8. Kaczka P, Michalczyk MM, Jastrząb R, et al. J Hum Kinet. 2019;68:211-222.

doi:10.2478/hukin-2019-0070

9. Vukovich MD, Stubbs NB, Bohlken RM. J Nutr. 2001;131(7):2049-2052.

doi:10.1093/jn/131.7.2049

10. Gallagher PM, Carrithers JA, Godard MP, et al. Med Sci Sports Exerc.

2000;32(12):2109-2115. doi:10.1097/00005768-200012000-00022

11. Deutz NE, Pereira SL, Hays NP, et al. Clin Nutr. 2013;32(5):704-712.

doi:10.1016/j.clnu.2013.02.011

12. Nissen SL, Sharp RL. J Appl Physio (1985). 2003;94(2):651-659. doi:10.1152/

japplphysiol.00755.2002

13. Bear DE, Langan A, Dimidi E, et al. Am J Clin Nutr. 2019;109(4):1119-1132.

doi:10.1093/ajcn/nqy373

14. Stockton KA, Mengersen K, Paratz JD, et al. Osteoporosis Int. 2011;22(3):859-

871. doi:10.1007/s00198-010-1407-y

15. Beaudart C, Buckinx F, Rabenda V, et al. J Clin Endocrinol Metab.

2014;99(11):4336-4345. doi:10.1210/jc.2014-1742

16. Ryan ZC, Craig TA, Folmes CD, et al. J Biol Chem. 2016;291(3):1514-1528.

doi:10.1074/jbc.M115.684399

17. Hildebrand RA, Miller B, Warren A, et al. Int J Sport Nutr Exerc Metab.

2016;26(6):558-564. doi:10.1123/ijsnem.2016-0052

18. Rathmacher JA, Pitchford LM, Khoo P, et al. J Gerontol A Biol Sci Med Sci.

2020;75(11):2089-2097. doi:10.1093/gerona/glaa218

19. Fuller JC Jr, Baier S, Flakoll P, et al. JPEN J Parented Enteral Nutr.

2011;35(6):757-762. doi:10.1177/0148607111413903